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  1. UGENE
  2. UGENE-1171

BWA/Bowtie alignment fails

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Critical
    • Resolution: Fixed
    • Affects Version/s: 1.11.1
    • Fix Version/s: 1.11.4, 1.12
    • Component/s: NGS
    • Labels:
    • Environment:

      Windows 7, 64 bit, i3 4GB memory

    • Tests Type:
      Manual scenario
    • Affect Type:
      Userdefined

      Description

      I tried to align Illumina FASTQ files to hg19. Bowtie/BWA indexes the reference genome just fine, but they stop afterwards with error code 1 (Bowtie) or code 3 (BWA), eg "Assembly task finished with error: Subtask

      {DnaAssemblyToRefTask}

      is failed: Subtask

      {Bowtie reads assembly}

      is failed: Subtask

      {Bowtie run task}

      is failed: Bowtie tool exited with code 1." (I downloaded the 64-bit external tools.)
      Thank you

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            People

            Assignee:
            ggrekhov German Grekhov
            Reporter:
            pgyarmati Peter Gyarmati
            Assigned Tester:
            Vladimir Malinovskiy
            Watchers:
            1 Start watching this issue

              Dates

              Created:
              Updated:
              Resolved: