Uploaded image for project: 'UGENE'
  1. UGENE
  2. UGENE-5162

It is impossible to use SAMtools out of the sample "Call variants with SAMtools"

    XMLWordPrintable

    Details

    • Type: Improvement
    • Status: Closed
    • Priority: Critical
    • Resolution: Fixed
    • Affects Version/s: 1.21
    • Fix Version/s: 1.23
    • Component/s: NGS, Workflow
    • Labels:
    • Story Points:
      1
    • Sprint:
      DEV-23-1, DEV-23-2
    • Affect Type:
      Userdefined

      Description

      The workflow element "Call Variants with SAMtools" is used in the sample with the same name. This sample is developed to be handy (with a wizard), it can process a lot of input datasets.

      But it is hard to use it (or even impossible) in a workflow: Aligner (BWA) -> Call variants.

      1. References. There are 2 input ports, and 1 of them is a reference port. But when you align with BWA different datasets of reads, the corresponding BWA element gets only 1 reference (as a parameter). This mismatch in references count doesn't let to use that workflow correctly.
      2. Datasets. BWA doesn't produce datasets (actually, it is a BWA bug). So, "Call variants with SAMtools" can't produce several output VCFs.

      A possible solution here is to create a parameter for reference input type: from a file or form a port. This parameter changes the number of ports in the element.

        Attachments

          Activity

            People

            Assignee:
            epushkova Eugenia Pushkova [X] (Inactive)
            Reporter:
            ggrekhov German Grekhov
            Assigned Tester:
            Kirill Rasputin
            Watchers:
            2 Start watching this issue

              Dates

              Created:
              Updated:
              Resolved: