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  1. UGENE
  2. UGENE-5529

FASTQ containing multiple sequences does not work with Sanger Analysis Workflow

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Blocker
    • Resolution: Won't Fix
    • Affects Version/s: 1.26
    • Fix Version/s: None
    • Component/s: Workflow
    • Labels:
      None
    • Affect Type:
      Userdefined

      Description

      As a workaround from this https://local.ugene.unipro.ru/tracker/browse/UGENE-3257 , I am now using FASTQ files provided by my sequencing service to analyze Sanger sequencing results.

      For one reference sequence, I have a FASTQ file containing all the Sanger reads + the associated quality values.

      When I add the file to the input of the "Trim and Align Sanger Reads" workflow, it doesn't work.

      But if I manually split the file to many FASTQ files (each one containing only one sequence + its quality values) and add all of them to the input of the workflow, it does work.

      It's a bit painful to have to process the FASTQ file before running the workflow.

      Do you know how I could fix that ?

        Attachments

        1. fastq_split.uwl
          1 kB
        2. fixed_sanger.uwl
          7 kB
        3. pFB7-CKAP5.fastq
          36 kB
        4. pFB7-CKAP5-USER-Cloning.gb
          15 kB

          Activity

            People

            Assignee:
            Unassigned
            Reporter:
            hadim hadim
            Watchers:
            2 Start watching this issue

              Dates

              Created:
              Updated:
              Resolved: