LOCUS       pJK590\minus\lag-2      4492 bp CIRCULAR                18-NOV-2012
SOURCE      
  ORGANISM  
BASE COUNT     1266 a       980 c      1004 g      1242 t 
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COMMENT                                   "{\\i Ava}I (367)" "@N (@S)" 5 13.4454 
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COMMENT                                   "{\\i Age}I (91)" "@N (@S)" 5 8.16357 10.454 
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COMMENT                                   "{\\i Kpn}I (92)" "@N (@S)" 5 10.4789 8.71165 
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COMMENT                                   "{\\i Pst}I (4481)" "@N (@S)" 5 -2.9616 
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COMMENT                                   "{\\i Nco}I (269)" "@N (@S)" 5 12.8908 
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COMMENT                                   10.454 0 -10 5.51978 0.924127 #158#)) 
COMMENT                                   "{\\i Hin}dIII (4465)" "@N (@S)" 5 -9.087 
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COMMENT                                   "{\\i Bam}HI (1)" "@N (@S)" 5 3.10923 17.4233 
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COMMENT                          "Created: 04/06/06 12:04PM" 1) 
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COMMENT        #7=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 269 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 4465 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 982 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1 0 0 "") 
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COMMENT         #0=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pJK590 lag 2" "" 0 0 1 
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COMMENT      (CGexDoc "pJK590 minus lag-2" 0 4492 
COMMENT     (SXF 
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     VNTAUTHOREML|emrutledge@nycap.rr.com|
COMMENT     VNTAUTHORNAME|Eric Rutledge|
COMMENT     VNTNAME|pJK590 minus lag-2|
COMMENT     LSOWNER|
COMMENT     VNTDBDATE|406987492|
COMMENT     VNTDATE|406987492|
COMMENT     ORIGDB|GenBank
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
EMBL_ID     pJK590\minus\lag-2        4492 bp    DNA     circular      6-APR-2006
UNIMARK     pJK590\minus\lag-2 features
            pJK590\minus\lag-2
FEATURES             Location/Qualifiers
     misc_feature    95..981
                     /5'term="AgeI"
                     /3'term="EcoRI"
                     /5'overhang="CCGG"
                     /3'overhang="AATT"
                     /5'strand="direct"
                     /3'strand="rev-compl"
                     /5'type="sticky"
                     /3'type="sticky"
                     /fragment_source="pJK590\minus\lag-2"
                     /ugene_name="Fragment 2"
                     /ugene_group="fragments"
     exon            103..273
                     /vntifkey=61
                     /label="1st\exon\GFP"
     exon            325..480
                     /vntifkey=61
                     /label="2nd\exon\GFP"
     exon            532..693
                     /vntifkey=61
                     /label="3rd\exon\GFP"
     exon            745..972
                     /vntifkey=61
                     /label="4th\exon\GFP\stop\codon"
     misc_feature    join(986..4492,1..90)
                     /5'term="EcoRI"
                     /3'term="AgeI"
                     /5'overhang="AATT"
                     /3'overhang="CCGG"
                     /5'strand="direct"
                     /3'strand="rev-compl"
                     /5'type="sticky"
                     /3'type="sticky"
                     /fragment_source="pJK590\minus\lag-2"
                     /ugene_name="Fragment 1"
                     /ugene_group="fragments"
ORIGIN
        1 GATCCCCGGG ATTGGCCAAA GGACCCAAAG GTATGTTTCG AATGATACTA ACATAACATA
       61 GAACATTTTC AGGAGGACCC TTGGAGGGTA CCGGTAGAAA AAATGAGTAA AGGAGAAGAA
      121 CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA
      181 TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG GAAAACTTAC CCTTAAATTT
      241 ATTTGCACTA CTGGAAAACT ACCTGTTCCA TGGGTAAGTT TAAACATATA TATACTAACT
      301 AACCCTGATT ATTTAAATTT TCAGCCAACA CTTGTCACTA CTTTCTGTTA TGGTGTTCAA
      361 TGCTTCTCGA GATACCCAGA TCATATGAAA CGGCATGACT TTTTCAAGAG TGCCATGCCC
      421 GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACGT
      481 AAGTTTAAAC AGTTCGGTAC TAACTAACCA TACATATTTA AATTTTCAGG TGCTGAAGTC
      541 AAGTTTGAAG GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA
      601 GATGGAAACA TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC
      661 ATGGCAGACA AACAAAAGAA TGGAATCAAA GTTGTAAGTT TAAACATGAT TTTACTAACT
      721 AACTAATCTG ATTTAAATTT TCAGAACTTC AAAATTAGAC ACAACATTGA AGATGGAAGC
      781 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCAATTG GCGATGGCCC TGTCCTTTTA
      841 CCAGACAACC ATTACCTGTC CACACAATCT GCCCTTTCGA AAGATCCCAA CGAAAAGAGA
      901 GACCACATGG TCCTTCTTGA GTTTGTAACA GCTGCTGGGA TTACACATGG CATGGATGAA
      961 CTATACAAAT AGCATTCGTA GAATTCCAAC TGAGCGCCGG TCGCTACCAT TACCAACTTG
     1021 TCTGGTGTCA AAAATAATAG GGGCCGCTGT CATCAGAGTA AGTTTAAACT GAGTTCTACT
     1081 AACTAACGAG TAATATTTAA ATTTTCAGCA TCTCGCGCCC GTGCCTCTGA CTTCTAAGTC
     1141 CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT GCTCCCACCC CCTATTTTTG
     1201 TTATTATCAA AAAAACTTCT TCTTAATTTC TTTGTTTTTT AGCTTCTTTT AAGTCACCTC
     1261 TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA GTCGAAAAAA
     1321 ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA ATGTTCTGTG
     1381 TACACTTCTT ATGTTTTTTT TACTTCTGAT AAATTTTTTT TGAAACATCA TAGAAAAAAC
     1441 CGCACACAAA ATACCTTATC ATATGTTACG TTTCAGTTTA TGACCGCAAT TTTTATTTCT
     1501 TCGCACGTCT GGGCCTCTCA TGACGTCAAA TCATGCTCAT CGTGAAAAAG TTTTGGAGTA
     1561 TTTTTGGAAT TTTTCAATCA AGTGAAAGTT TATGAAATTA ATTTTCCTGC TTTTGCTTTT
     1621 TGGGGGTTTC CCCTATTGTT TGTCAAGAGT TTCGAGGACG GCGTTTTTCT TGCTAAAATC
     1681 ACAAGTATTG ATGAGCACGA TGCAAGAAAG ATCGGAAGAA GGTTTGGGTT TGAGGCTCAG
     1741 TGGAAGGTGA GTAGAAGTTG ATAATTTGAA AGTGGAGTAG TGTCTATGGG GTTTTTGCCT
     1801 TAAATGACAG AATACATTCC CAATATACCA AACATAACTG TTTCCTACTA GTCGGCCGTA
     1861 CGGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT
     1921 CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG
     1981 CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT
     2041 TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA
     2101 CCGCATCAGG CGGCCTTAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA
     2161 TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA
     2221 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT
     2281 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC
     2341 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA
     2401 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA
     2461 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT
     2521 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG
     2581 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC
     2641 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA
     2701 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT
     2761 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG
     2821 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA
     2881 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG
     2941 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG
     3001 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG
     3061 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG
     3121 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG
     3181 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA
     3241 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
     3301 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC
     3361 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC
     3421 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC
     3481 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC
     3541 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT
     3601 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT
     3661 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT
     3721 ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT
     3781 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG
     3841 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT
     3901 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT
     3961 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG
     4021 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG
     4081 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC
     4141 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG
     4201 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC
     4261 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG
     4321 GAAACAGCTA TGACCATGAT TACGCCAAGC TGTAAGTTTA AACATGATCT TACTAACTAA
     4381 CTATTCTCAT TTAAATTTTC AGAGCTTAAA AATGGCTGAA ATCACTCACA ACGATGGATA
     4441 CGCTAACAAC TTGGAAATGA AATAAGCTTG CATGCCTGCA GGTCGACTAG AG
//
