LOCUS       YCpLAC36\pGAL-PR        7885 bp    DNA     circular     20-JUN-2010
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|557530498|
COMMENT     VNTDBDATE|557530498|
COMMENT     LSOWNER|
COMMENT     VNTNAME|YCpLAC36 pGAL-PRE1-mycDAM-tCYC1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Stepan|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1709 0 0 "") 
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COMMENT        #8=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1366 0 0 "") 
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COMMENT        #10=(CRSite (CStringList) "XbaI" "tctaga" 2 0 0 0 "") 
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COMMENT        #25=(CFSignal (CObList) "YCpLAC33 near NheI top" 21 0 0 4032 4054 0 
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FEATURES             Location/Qualifiers
     CDS             complement(4819..5619)
                     /vntifkey="4"
                     /label=Ura3
     centromere      3309..3368
                     /vntifkey="5"
                     /label=Cen4
     misc_feature    2759..2873
                     /vntifkey="21"
                     /label=rmd1
                     /note="without 5'"
     misc_feature    complement(4320..4709)
                     /vntifkey="21"
                     /label=trp1
                     /note="without 5'"
     CDS             6084..6941
                     /vntifkey="4"
                     /label=AMPr
     rep_origin      7690..7714
                     /vntifkey="33"
                     /label=ori
     misc_feature    162..184
                     /vntifkey="21"
                     /label=YCpLAC33\MCS\top
     misc_feature    2590..2612
                     /vntifkey="21"
                     /label=YCpLAC33\MCS\bottom
     misc_feature    5746..5766
                     /vntifkey="21"
                     /label=YCpLAC33\near\NdeI\bott
     misc_feature    4032..4054
                     /vntifkey="21"
                     /label=YCpLAC33\near\NheI\top
     misc_feature    5185..5204
                     /vntifkey="21"
                     /label=URA-RT-bottom
     misc_feature    5659..5680
                     /vntifkey="21"
                     /label=Near\PstI\URA3\top
     misc_feature    5704..5731
                     /vntifkey="21"
                     /label=Nde\I\del\oligo
     misc_feature    5409..5414
                     /vntifkey="21"
                     /label=Nco\del\mutation
     misc_feature    239..766
                     /vntifkey="21"
                     /label=pGAL
     misc_feature    2246..2501
                     /vntifkey="21"
                     /label=tCYC1
     misc_feature    1361..2239
                     /vntifkey="21"
                     /label=Myc-DAM
     misc_feature    767..1360
                     /vntifkey="21"
                     /label=PRE1
BASE COUNT     2250 a      1720 c      1637 g      2278 t 
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttca 
      241 gctttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa ctgctcattg 
      301 ctatattgaa gtacggatta gaagccgccg agcgggtgac agccctccga aggaagactc 
      361 tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc 
      421 gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa 
      481 attggcagta acctggcccc acaaaccttc aaatgaacga atcaaattaa caaccatagg 
      541 atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga 
      601 tttttgatct attaacagat atataaatgc aaaaactgca taaccacttt aactaatact 
      661 ttcaacattt tcggtttgta ttacttctta ttcaaatgta ataaaagtat caacaaaaaa 
      721 ttgttaatat acctctatac tttaacgtca aggagaaaaa actaccatgg atattattct 
      781 gggcatccgt gtacaggatt ctgtcattct agcgtcttct aaggcagtca caagaggtat 
      841 ttctgtttta aaagattctg atgataaaac gagacaatta tcgccacata cattgatgag 
      901 ttttgccggt gaagctggtg acaccgttca attcgccgag tacattcaag ccaatatcca 
      961 attatactcc attagagaag attatgagct ctctccacaa gcagtatcta gttttgttag 
     1021 gcaagaatta gccaagtcaa ttaggtcgag aagaccatac caagtcaacg tattgattgg 
     1081 aggctatgac aaaaagaaga acaaaccgga actatatcaa attgactact tgggtactaa 
     1141 agtcgaatta ccctatggtg ctcatggtta ctcggggttt tacacattct ctttactaga 
     1201 tcatcattat agacctgata tgactactga ggagggttta gatttattaa aactatgtgt 
     1261 acaagagctt gaaaaaagaa tgccaatgga cttcaagggc gtcattgtta aaatcgtgga 
     1321 taaagatggc ataagacaag tagatgactt ccaggcacag ctgcaggaac agaaactcat 
     1381 ctctgaagag gatctggcac ccggcaagaa aaatcgcgct tttttgaagt gggcaggggg 
     1441 caagtatccc ctgcttgatg atattaaacg gcatttgccc aagggcgaat gtctggttga 
     1501 gccttttgta ggtgccgggt cggtgtttct caacaccgac ttttctcgtt acatccttgc 
     1561 cgatatcaat agcgacctga tcagtctcta taacattgtg aagatgcgta ctgatgagta 
     1621 cgtacaggcc gcacgcgagc tgtttgttcc cgaaacaaat tgcgccgagg tttactatca 
     1681 gttccgcgaa gagttcaaca aaagccagga tccgttccgt cgggcggtac tgtttttata 
     1741 tttgaaccgc tacggttaca acggcctgtg tcgttacaat ctgcgcggtg agtttaacgt 
     1801 gccgttcggc cgctacaaaa aaccctattt cccggaagca gagttgtatc acttcgctga 
     1861 aaaagcgcag aatgcctttt tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga 
     1921 tgatgcatcc gtcgtctatt gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt 
     1981 tacggcgtat cacacaaaca gttttacgct tgaacaacaa gcgcatctgg cggagatcgc 
     2041 cgaaggtctg gttgagcgcc atattccagt gctgatctcc aatcacgata cgatgttaac 
     2101 gcgtgagtgg tatcagcgcg caaaattgca tgtcgtcaaa gttcgacgca gtataagcag 
     2161 caacggcggc acacgtaaaa aggtggacga actgctggct ttgtacaaac caggagtcgt 
     2221 ttcacccgcg aaaaaataac tcgagatcat gtaattagtt atgtcacgct tacattcacg 
     2281 ccctcctccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt 
     2341 ccctatttat tttttttaat agttatgtta gtattaagaa cgttatttat atttcaaatt 
     2401 tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt 
     2461 gagaaggttt tgggacgctc gaaggcttta atttgcaagc tggtaccgag ctcgaatttt 
     2521 tctctcgtac cgagctcaaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 
     2581 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 
     2641 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 
     2701 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatcgc 
     2761 tgggccattc tcatgaagaa tatcttgaat ttattgtcat attactagtt ggtgtggaag 
     2821 tccatatatc ggtgatcaat atagtggttg acatgctggc tagtcaacat tgagcctttt 
     2881 gatcatgcaa atatattacg gtattttaca atcaaatatc aaacttaact attgacttta 
     2941 taacttattt aggtggtaac attcttataa aaaagaaaaa aattactgca aaacagtact 
     3001 agcttttaac ttgtatccta ggttatctat gctgtctcac catagagaat attacctatt 
     3061 tcagaatgta tgtccatgat tcgccgggta aatacatata atacacaaat ctggcttaat 
     3121 aaagtctata atatatctca taaagaagtg ctaaattggc tagtgctata tatttttaag 
     3181 aaaatttctt ttgactaagt ccatatcgac tttgtaaaag ttcactttag catacatata 
     3241 ttacacgagc cagaaattgt aacttttgcc taaaatcaca aattgcaaaa tttaattgct 
     3301 tgcaaaaggt cacatgctta taatcaactt ttttaaaaat ttaaaatact tttttatttt 
     3361 ttatttttaa acataaatga aataatttat ttattgttta tgattaccga aacataaaac 
     3421 ctgctcaaga aaaagaaact gttttgtcct tggaaaaaaa gcactaccta ggagcggcca 
     3481 aaatgccgag gctttcatag cttaaactct ttacagaaaa taggcattat agatcagttc 
     3541 gagttttctt attcttcctt ccggttttat cgtcacagtt ttacagtaaa taagtatcac 
     3601 ctcttagagt tcgatgataa gctgtcaaac atgagaatta attccacatg ttaaaatagt 
     3661 gaaggagcat gttcggcaca cagtggaccg aacgtggggt aagtgcacta gggtccggtt 
     3721 aaacggatct cgcattgatg aggcaacgct aattatcaac atatagattg ttatctatct 
     3781 gcatgaacac gaaatcttta cttgacgact tgaggctgat ggtgtttatg caaagaaacc 
     3841 actgtgttta atatgtgtca ctgtttgata ttactgtcag cgtagaagat aatagtaaaa 
     3901 gcggttaata agtgtatttg agataagtgt gataaagttt ttacagcgaa aagacgataa 
     3961 atacaagaaa atgattacga ggatacggag agaggtatgt acatgtgtat ttatatacta 
     4021 agctgccggc ggttgtttgc aagaccgaga aaaggctagc aagaatcggg tcattgtagc 
     4081 gtatgcgcct gtgaacattc tcttcaacaa gtttgattcc attgcggtga aatggtaaaa 
     4141 gtcaaccccc tgcgatgtat attttcctgt acaatcaatc aaaaagccaa atgatttagc 
     4201 attatcttta catcttgtta ttttacagat tttatgttta gatcttttat gcttgctttt 
     4261 caaaaggctt gcaggcaagt gcacaaacaa tacttaaata aatactactc agtaataacc 
     4321 tatttcttag catttttgac gaaatttgct attttgttag agtcttttac accatttgtc 
     4381 tccacacctc cgcttacatc aacaccaata acgccattta atctaagcgc atcaccaaca 
     4441 ttttctggcg tcagtccacc agctaacata aaatgtaagc tctcggggct ctcttgcctt 
     4501 ccaacccagt cagaaatcga gttccaatcc aaaagttcac ctgtcccacc tgcttctgaa 
     4561 tcaaacaagg gaataaacga atgaggtttc tgtgaagctg cactgagtag tatgttgcag 
     4621 tcttttggaa atacgagtct tttaataact ggcaaaccga ggaactcttg gtattcttgc 
     4681 cacgactcat ctccatgcag ttggacgatc gatgataagc tgtcaaacat gagaattggg 
     4741 taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac 
     4801 ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc 
     4861 ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc 
     4921 aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc 
     4981 caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc 
     5041 ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct 
     5101 cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga 
     5161 caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt 
     5221 caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc 
     5281 tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa 
     5341 ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac 
     5401 tgtgccctcc attgaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt 
     5461 gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc 
     5521 acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa caggactagg 
     5581 atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc gtttcctgca 
     5641 tgtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt cttcaacact 
     5701 acatatcggt atatatacgt ggtgttgaag atatgcgtat atataccaat ctaagtctgt 
     5761 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaagaa 
     5821 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaattgaaaa gctaattctt 
     5881 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 
     5941 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 
     6001 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 
     6061 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 
     6121 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 
     6181 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 
     6241 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 
     6301 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 
     6361 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 
     6421 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 
     6481 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 
     6541 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 
     6601 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 
     6661 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 
     6721 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 
     6781 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 
     6841 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 
     6901 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 
     6961 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 
     7021 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 
     7081 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 
     7141 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 
     7201 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 
     7261 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 
     7321 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 
     7381 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 
     7441 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 
     7501 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 
     7561 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 
     7621 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 
     7681 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 
     7741 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 
     7801 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 
     7861 agtcagtgag cgaggaagcg gaaga 
//
