LOCUS       YCpLAC36\p              7921 bp    CIRCULAR             23-JUN-2014
SOURCE      
  ORGANISM  
BASE COUNT     2255 a      1731 c      1646 g      2289 t 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT         "NcoI" "PstI" "SmaI" "XbaI" "XhoI" "XmaI" "NdeI" "NheI" "NotI") 
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COMMENT      (CGexDoc "YCpLAC36 pGAL-NESP-PRE1-mycDAM-tCYC1" 0 7921 
COMMENT     (SXF 
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTOAUTHORNAME|Stepan|
COMMENT     VNTAUTHORNAME|aleom|
COMMENT     VNTNAME|YCpLAC36 pGAL-NESP-PRE1-mycDAM-tCYC1|
COMMENT     VNTDBDATE|701196421|
COMMENT     VNTDATE|701196421|
COMMENT     ORIGDB|GenBank
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
EMBL_ID     YCpLAC36\p   7921 bp    DNA   circular            19-JUN-2014
UNIMARK     YCpLAC36\p features
            YCpLAC36\p
FEATURES             Location/Qualifiers
     misc_feature    162..184
                     /vntifkey=21
                     /label="YCpLAC33\MCS\top"
     misc_feature    239..764
                     /ugene_name="pGAL1"
                     /ugene_group="promoter"
     misc_feature    766..801
                     /vntifkey=21
                     /label="NESP-oligo"
     misc_feature    803..1396
                     /vntifkey=21
                     /label="PRE1"
     misc_feature    1397..2275
                     /vntifkey=21
                     /label="Myc-DAM"
     misc_feature    2282..2537
                     /vntifkey=21
                     /label="tCYC1"
     misc_feature    2626..2648
                     /vntifkey=21
                     /label="YCpLAC33\MCS\bottom"
     misc_feature    2795..2909
                     /vntifkey=21
                     /label="rmd1"
                     /note="without 5'"
     misc_feature    3345..3404
                     /vntifkey=5
                     /label="Cen4"
                     /ugene_name="centromere"
     misc_feature    4068..4090
                     /vntifkey=21
                     /label="YCpLAC33\near\NheI\top"
     misc_feature    complement(4356..4745)
                     /vntifkey=21
                     /label="trp1"
                     /note="without 5'"
     CDS             complement(4855..5655)
                     /vntifkey=4
                     /label="Ura3"
     misc_feature    5221..5240
                     /vntifkey=21
                     /label="URA-RT-bottom"
     misc_feature    5445..5450
                     /vntifkey=21
                     /label="Nco\del\mutation"
     misc_feature    5695..5716
                     /vntifkey=21
                     /label="Near\PstI\URA3\top"
     misc_feature    5740..5767
                     /vntifkey=21
                     /label="Nde\I\del\oligo"
     misc_feature    5782..5802
                     /vntifkey=21
                     /label="YCpLAC33\near\NdeI\bott"
     CDS             6120..6977
                     /vntifkey=4
                     /label="AMPr"
     rep_origin      7726..7750
                     /vntifkey=33
                     /label="ori"
ORIGIN
        1 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
       61 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT
      121 CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT
      181 TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTTCA
      241 GCTTTAGCCT AAAAAAACCT TCTCTTTGGA ACTTTCAGTA ATACGCTTAA CTGCTCATTG
      301 CTATATTGAA GTACGGATTA GAAGCCGCCG AGCGGGTGAC AGCCCTCCGA AGGAAGACTC
      361 TCCTCCGTGC GTCCTCGTCT TCACCGGTCG CGTTCCTGAA ACGCAGATGT GCCTCGCGCC
      421 GCACTGCTCC GAACAATAAA GATTCTACAA TACTAGCTTT TATGGTTATG AAGAGGAAAA
      481 ATTGGCAGTA ACCTGGCCCC ACAAACCTTC AAATGAACGA ATCAAATTAA CAACCATAGG
      541 ATGATAATGC GATTAGTTTT TTAGCCTTAT TTCTGGGGTA ATTAATCAGC GAAGCGATGA
      601 TTTTTGATCT ATTAACAGAT ATATAAATGC AAAAACTGCA TAACCACTTT AACTAATACT
      661 TTCAACATTT TCGGTTTGTA TTACTTCTTA TTCAAATGTA ATAAAAGTAT CAACAAAAAA
      721 TTGTTAATAT ACCTCTATAC TTTAACGTCA AGGAGAAAAA ACTACCATGG GCGAATTCTC
      781 TGTCGACTCT CTGCAGCTTC GCATGGATAT TATTCTGGGC ATCCGTGTAC AGGATTCTGT
      841 CATTCTAGCG TCTTCTAAGG CAGTCACAAG AGGTATTTCT GTTTTAAAAG ATTCTGATGA
      901 TAAAACGAGA CAATTATCGC CACATACATT GATGAGTTTT GCCGGTGAAG CTGGTGACAC
      961 CGTTCAATTC GCCGAGTACA TTCAAGCCAA TATCCAATTA TACTCCATTA GAGAAGATTA
     1021 TGAGCTCTCT CCACAAGCAG TATCTAGTTT TGTTAGGCAA GAATTAGCCA AGTCAATTAG
     1081 GTCGAGAAGA CCATACCAAG TCAACGTATT GATTGGAGGC TATGACAAAA AGAAGAACAA
     1141 ACCGGAACTA TATCAAATTG ACTACTTGGG TACTAAAGTC GAATTACCCT ATGGTGCTCA
     1201 TGGTTACTCG GGGTTTTACA CATTCTCTTT ACTAGATCAT CATTATAGAC CTGATATGAC
     1261 TACTGAGGAG GGTTTAGATT TATTAAAACT ATGTGTACAA GAGCTTGAAA AAAGAATGCC
     1321 AATGGACTTC AAGGGCGTCA TTGTTAAAAT CGTGGATAAA GATGGCATAA GACAAGTAGA
     1381 TGACTTCCAG GCACAGCTGC AGGAACAGAA ACTCATCTCT GAAGAGGATC TGGCACCCGG
     1441 CAAGAAAAAT CGCGCTTTTT TGAAGTGGGC AGGGGGCAAG TATCCCCTGC TTGATGATAT
     1501 TAAACGGCAT TTGCCCAAGG GCGAATGTCT GGTTGAGCCT TTTGTAGGTG CCGGGTCGGT
     1561 GTTTCTCAAC ACCGACTTTT CTCGTTACAT CCTTGCCGAT ATCAATAGCG ACCTGATCAG
     1621 TCTCTATAAC ATTGTGAAGA TGCGTACTGA TGAGTACGTA CAGGCCGCAC GCGAGCTGTT
     1681 TGTTCCCGAA ACAAATTGCG CCGAGGTTTA CTATCAGTTC CGCGAAGAGT TCAACAAAAG
     1741 CCAGGATCCG TTCCGTCGGG CGGTACTGTT TTTATATTTG AACCGCTACG GTTACAACGG
     1801 CCTGTGTCGT TACAATCTGC GCGGTGAGTT TAACGTGCCG TTCGGCCGCT ACAAAAAACC
     1861 CTATTTCCCG GAAGCAGAGT TGTATCACTT CGCTGAAAAA GCGCAGAATG CCTTTTTCTA
     1921 TTGTGAGTCT TACGCCGATA GCATGGCGCG CGCAGATGAT GCATCCGTCG TCTATTGCGA
     1981 TCCGCCTTAT GCACCGCTGT CTGCGACCGC CAACTTTACG GCGTATCACA CAAACAGTTT
     2041 TACGCTTGAA CAACAAGCGC ATCTGGCGGA GATCGCCGAA GGTCTGGTTG AGCGCCATAT
     2101 TCCAGTGCTG ATCTCCAATC ACGATACGAT GTTAACGCGT GAGTGGTATC AGCGCGCAAA
     2161 ATTGCATGTC GTCAAAGTTC GACGCAGTAT AAGCAGCAAC GGCGGCACAC GTAAAAAGGT
     2221 GGACGAACTG CTGGCTTTGT ACAAACCAGG AGTCGTTTCA CCCGCGAAAA AATAACTCGA
     2281 GATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCTCCCACAT CCGCTCTAAC
     2341 CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTTAATAGTT
     2401 ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAAACGC
     2461 GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG
     2521 GCTTTAATTT GCAAGCTGGT ACCGAGCTCG AATTTTTCTC TCGTACCGAG CTCAAATTCA
     2581 CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC
     2641 CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC
     2701 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT
     2761 ACGCATCTGT GCGGTATTTC ACACCGCATA TATCGCTGGG CCATTCTCAT GAAGAATATC
     2821 TTGAATTTAT TGTCATATTA CTAGTTGGTG TGGAAGTCCA TATATCGGTG ATCAATATAG
     2881 TGGTTGACAT GCTGGCTAGT CAACATTGAG CCTTTTGATC ATGCAAATAT ATTACGGTAT
     2941 TTTACAATCA AATATCAAAC TTAACTATTG ACTTTATAAC TTATTTAGGT GGTAACATTC
     3001 TTATAAAAAA GAAAAAAATT ACTGCAAAAC AGTACTAGCT TTTAACTTGT ATCCTAGGTT
     3061 ATCTATGCTG TCTCACCATA GAGAATATTA CCTATTTCAG AATGTATGTC CATGATTCGC
     3121 CGGGTAAATA CATATAATAC ACAAATCTGG CTTAATAAAG TCTATAATAT ATCTCATAAA
     3181 GAAGTGCTAA ATTGGCTAGT GCTATATATT TTTAAGAAAA TTTCTTTTGA CTAAGTCCAT
     3241 ATCGACTTTG TAAAAGTTCA CTTTAGCATA CATATATTAC ACGAGCCAGA AATTGTAACT
     3301 TTTGCCTAAA ATCACAAATT GCAAAATTTA ATTGCTTGCA AAAGGTCACA TGCTTATAAT
     3361 CAACTTTTTT AAAAATTTAA AATACTTTTT TATTTTTTAT TTTTAAACAT AAATGAAATA
     3421 ATTTATTTAT TGTTTATGAT TACCGAAACA TAAAACCTGC TCAAGAAAAA GAAACTGTTT
     3481 TGTCCTTGGA AAAAAAGCAC TACCTAGGAG CGGCCAAAAT GCCGAGGCTT TCATAGCTTA
     3541 AACTCTTTAC AGAAAATAGG CATTATAGAT CAGTTCGAGT TTTCTTATTC TTCCTTCCGG
     3601 TTTTATCGTC ACAGTTTTAC AGTAAATAAG TATCACCTCT TAGAGTTCGA TGATAAGCTG
     3661 TCAAACATGA GAATTAATTC CACATGTTAA AATAGTGAAG GAGCATGTTC GGCACACAGT
     3721 GGACCGAACG TGGGGTAAGT GCACTAGGGT CCGGTTAAAC GGATCTCGCA TTGATGAGGC
     3781 AACGCTAATT ATCAACATAT AGATTGTTAT CTATCTGCAT GAACACGAAA TCTTTACTTG
     3841 ACGACTTGAG GCTGATGGTG TTTATGCAAA GAAACCACTG TGTTTAATAT GTGTCACTGT
     3901 TTGATATTAC TGTCAGCGTA GAAGATAATA GTAAAAGCGG TTAATAAGTG TATTTGAGAT
     3961 AAGTGTGATA AAGTTTTTAC AGCGAAAAGA CGATAAATAC AAGAAAATGA TTACGAGGAT
     4021 ACGGAGAGAG GTATGTACAT GTGTATTTAT ATACTAAGCT GCCGGCGGTT GTTTGCAAGA
     4081 CCGAGAAAAG GCTAGCAAGA ATCGGGTCAT TGTAGCGTAT GCGCCTGTGA ACATTCTCTT
     4141 CAACAAGTTT GATTCCATTG CGGTGAAATG GTAAAAGTCA ACCCCCTGCG ATGTATATTT
     4201 TCCTGTACAA TCAATCAAAA AGCCAAATGA TTTAGCATTA TCTTTACATC TTGTTATTTT
     4261 ACAGATTTTA TGTTTAGATC TTTTATGCTT GCTTTTCAAA AGGCTTGCAG GCAAGTGCAC
     4321 AAACAATACT TAAATAAATA CTACTCAGTA ATAACCTATT TCTTAGCATT TTTGACGAAA
     4381 TTTGCTATTT TGTTAGAGTC TTTTACACCA TTTGTCTCCA CACCTCCGCT TACATCAACA
     4441 CCAATAACGC CATTTAATCT AAGCGCATCA CCAACATTTT CTGGCGTCAG TCCACCAGCT
     4501 AACATAAAAT GTAAGCTCTC GGGGCTCTCT TGCCTTCCAA CCCAGTCAGA AATCGAGTTC
     4561 CAATCCAAAA GTTCACCTGT CCCACCTGCT TCTGAATCAA ACAAGGGAAT AAACGAATGA
     4621 GGTTTCTGTG AAGCTGCACT GAGTAGTATG TTGCAGTCTT TTGGAAATAC GAGTCTTTTA
     4681 ATAACTGGCA AACCGAGGAA CTCTTGGTAT TCTTGCCACG ACTCATCTCC ATGCAGTTGG
     4741 ACGATCGATG ATAAGCTGTC AAACATGAGA ATTGGGTAAT AACTGATATA ATTAAATTGA
     4801 AGCTCTAATT TGTGAGTTTA GTATACATGC ATTTACTTAT AATACAGTTT TTTAGTTTTG
     4861 CTGGCCGCAT CTTCTCAAAT ATGCTTCCCA GCCTGCTTTT CTGTAACGTT CACCCTCTAC
     4921 CTTAGCATCC CTTCCCTTTG CAAATAGTCC TCTTCCAACA ATAATAATGT CAGATCCTGT
     4981 AGAGACCACA TCATCCACGG TTCTATACTG TTGACCCAAT GCGTCTCCCT TGTCATCTAA
     5041 ACCCACACCG GGTGTCATAA TCAACCAATC GTAACCTTCA TCTCTTCCAC CCATGTCTCT
     5101 TTGAGCAATA AAGCCGATAA CAAAATCTTT GTCGCTCTTC GCAATGTCAA CAGTACCCTT
     5161 AGTATATTCT CCAGTAGATA GGGAGCCCTT GCATGACAAT TCTGCTAACA TCAAAAGGCC
     5221 TCTAGGTTCC TTTGTTACTT CTTCTGCCGC CTGCTTCAAA CCGCTAACAA TACCTGGGCC
     5281 CACCACACCG TGTGCATTCG TAATGTCTGC CCATTCTGCT ATTCTGTATA CACCCGCAGA
     5341 GTACTGCAAT TTGACTGTAT TACCAATGTC AGCAAATTTT CTGTCTTCGA AGAGTAAAAA
     5401 ATTGTACTTG GCGGATAATG CCTTTAGCGG CTTAACTGTG CCCTCCATTG AAAAATCAGT
     5461 CAAGATATCC ACATGTGTTT TTAGTAAACA AATTTTGGGA CCTAATGCTT CAACTAACTC
     5521 CAGTAATTCC TTGGTGGTAC GAACATCCAA TGAAGCACAC AAGTTTGTTT GCTTTTCGTG
     5581 CATGATATTA AATAGCTTGG CAGCAACAGG ACTAGGATGA GTAGCAGCAC GTTCCTTATA
     5641 TGTAGCTTTC GACATGATTT ATCTTCGTTT CCTGCATGTT TTTGTTCTGT GCAGTTGGGT
     5701 TAAGAATACT GGGCAATTTC ATGTTTCTTC AACACTACAT ATCGGTATAT ATACGTGGTG
     5761 TTGAAGATAT GCGTATATAT ACCAATCTAA GTCTGTGCTC CTTCCTTCGT TCTTCCTTCT
     5821 GTTCGGAGAT TACCGAATCA AAAAAATTTC AAAGAAACCG AAATCAAAAA AAAGAATAAA
     5881 AAAAAAATGA TGAATTGAAT TGAAAAGCTA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC
     5941 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT
     6001 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
     6061 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
     6121 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
     6181 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
     6241 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
     6301 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
     6361 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
     6421 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
     6481 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
     6541 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
     6601 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
     6661 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
     6721 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
     6781 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
     6841 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
     6901 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
     6961 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
     7021 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
     7081 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     7141 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     7201 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     7261 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     7321 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     7381 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     7441 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     7501 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     7561 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     7621 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     7681 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     7741 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     7801 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     7861 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     7921 A
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