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  1. UGENE
  2. UGENE-7442

The reverse sequences in Task report [PCR Primer Design For DNA Assembly Task] should be taken from complementary sequence

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Major
    • Resolution: Fixed
    • Affects Version/s: None
    • Fix Version/s: None
    • Component/s: None
    • Labels:
    • Tests Type:
      GUI automatic
    • Sprint:
      DEV-41-3, DEV-41-4
    • Affect Type:
      Userdefined

      Description

      1. Open _common_data/pcr_primer_design/backbone.fa
      2. Open PCR Primer Design for DNA Assembly Tab.
      3. Set Parameters of priming sequences -> Melting point -> 48.
      4. Start. Wait for task to finish.
      5. Open report.
        Expected: A Reverse sequence is "AAGGGTATCACCTTCAAACTT" (same as annotation table).
        Current: A Reverse is "AAGTTTGAAGGTGATACCCTT" (same area as annotation, but derived from forward rather than complementary)

      Likewise for all other reverse sequences.

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              Assignee:
              biserova Evelina
              Reporter:
              biserova Evelina
              Assigned Tester:
              Dmitrii Sukhomlinov
              Watchers:
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                Dates

                Created:
                Updated:
                Resolved: