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  1. UGENE
  2. UGENE-7813

Primer3 calculator shows incorrect Tm for short sequences.

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    Details

    • Type: Bug
    • Status: Closed
    • Priority: Major
    • Resolution: Fixed
    • Affects Version/s: 46
    • Fix Version/s: 47
    • Component/s: None
    • Labels:
      None
    • Sprint:
      DEV-47-1, DEV-47-2
    • Affect Type:
      Userdefined

      Description

      1. Open HumanT1
      2. Select base at position 51
      3. Switch to Primer3 Tm algorithm

      The melting temperature is -437C
      If you select 2 letters: 51 & 52 the temperature is -177C

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            Assignee:
            yalgaer Yuliya Algaer
            Reporter:
            yalgaer Yuliya Algaer
            Assigned Tester:
            Svetlana Samoilenko
            Watchers:
            2 Start watching this issue

              Dates

              Created:
              Updated:
              Resolved: