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  1. UGENE
  2. UGENE-7889

Add enzyme's overhangs and the corresponding filter

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    Details

    • Type: Improvement
    • Status: Closed
    • Priority: Major
    • Resolution: Fixed
    • Affects Version/s: None
    • Fix Version/s: 48
    • Component/s: None
    • Sprint:
      DEV-48-1, DEV-48-2, DEV-48-3
    • Affect Type:
      Userdefined

      Description

      Each enzyme could have a certain types of overhangs (sometimes one site has several of them):

      • Blunt - the cut is located in the middle of the site.
      • Sticky - the cut is locate anywhere but the middle of the site.
      • Nondegenerate sticky - the save as previous, but the overhang between cuts has only A, C, G or T  (no extended) symbols.
      • 5' - the cut of the forward strand is to the left of the cut of the reverse-complementary strand.
      • 3' - the cut of the forward strand is to the right of the cut of the reverse-complementary strand (see sticky image).
      • Single-strand nick - cut only on one strand (not sure, that our database has this type). They are not just "Sticky"

      Also we need to add two mixed points:

      • Blunt and sticky. If there are no "Single-strand nick" in our database, it will be "Blunt and sticky (Any)".
      • Blunt and nondegenerate sticky.

        Attachments

        1. image-2023-05-27-18-02-52-976.png
          23 kB
          Dmitrii Sukhomlinov
        2. image-2023-05-27-18-03-27-895.png
          23 kB
          Dmitrii Sukhomlinov
        3. image-2023-05-27-18-03-36-121.png
          20 kB
          Dmitrii Sukhomlinov
        4. image-2023-05-27-18-06-36-523.png
          16 kB
          Dmitrii Sukhomlinov
        5. image-2023-05-27-18-09-39-272.png
          15 kB
          Dmitrii Sukhomlinov
        6. image-2023-05-27-18-15-00-575.png
          43 kB
          Dmitrii Sukhomlinov

          Activity

            People

            Assignee:
            dsukhomlinov Dmitrii Sukhomlinov
            Reporter:
            dsukhomlinov Dmitrii Sukhomlinov
            Watchers:
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              Dates

              Created:
              Updated:
              Resolved: